Table 3.
AgeCGs and nonageCGs affiliated with gene expression within tissues classified by CpG context a
|
AgeCGs/genesb |
Non-ageCGs/genesc |
P
-valued |
|||
|---|---|---|---|---|---|
| Expressed | Non-expressed | Expressed | Non-expressed | ||
|
Blood |
|
|
|
|
|
| All |
271 (46.2) |
315 (53.8) |
4610 (78.4) |
1270 (21.6) |
<2.2 × 10-16 * |
| Island only |
121 (36.1) |
214 (63.9) |
2251 (89.0) |
277 (11.0) |
<2.2 × 10-16 * |
| Shore only |
101 (72.7) |
38 (27.3) |
1610 (83.5) |
317 (16.5) |
1.5 × 10-3 * |
| Other only |
49 (43.8) |
63 (56.2) |
749 (52.6) |
676 (47.4) |
0.09 |
|
Brain |
|
|
|
|
|
| All |
591 (78.8) |
159 (21.2) |
4404 (89.3) |
528 (10.7) |
3.5 × 10-16 * |
| Island only |
328 (79.4) |
85 (20.6) |
2243 (97.0) |
70 (3.0) |
<2.2 × 10-16 * |
| Shore only |
184 (85.6) |
31 (14.4) |
1564 (95.0) |
82 (5.0) |
1.2 × 10-7 * |
| Other only |
79 (64.8) |
43 (35.2) |
597 (61.4) |
376 (38.6) |
0.53 |
|
Kidney |
|
|
|
|
|
| All |
1209 (77.4) |
354 (22.6) |
4046 (89.9) |
456 (10.1) |
<2.2 × 10-16 * |
| Island only |
402 (71.2) |
163 (28.8) |
2089 (97.5) |
54 (2.5) |
<2.2 × 10-16 * |
| Shore only |
440 (85.8) |
73 (14.2) |
1362 (95.0) |
71 (5.0) |
1.1 × 10-11 * |
| Other onlye |
367 (75.7) |
118 (24.3) |
595 (64.3) |
331(35.7) |
1.6 × 10-5 * |
|
Muscle |
|
|
|
|
|
| Allf |
515 (77.2) |
152 (22.8) |
2784 (71.8) |
1095 (28.2) |
4.2 × 10-3 |
| Island only |
258 (78.4) |
71 (21.6) |
1262 (87.3) |
184 (12.7) |
5.2 × 10-5 * |
| Shore only |
142 (86.1) |
23 (13.9) |
1065 (82.0) |
233 (18.0) |
0.24 |
| Other only | 115 (66.5) | 58 (33.5) | 457 (40.3) | 678 (59.7) | 1.6 × 10-10 * |
aExpressed genes, FPKM > 0.25; non-expressed genes, FPKM < 0.05. Parentheses indicate percentage of total in each CpG context category.
b,cGenes in proximity to an ageCG or non-ageCG according to the smallest age-associated P-value for representative genes in each tissue.
dResulting P-values from Pearson’s Chi-squared tests between numbers of ageCGs and non-ageCGs within each classification.
eAgeCGs/genes found within CGOs from kidney showed a significantly greater number of expressed genes compared to non-ageCGs in kidney.
fMuscle was the only tissue that showed a greater number of total ageCGs/genes expressed compared to non-ageCGs/genes regardless of CpG contextthis same effect was observed within the CGO category.
*Significant P-values meet Bonferroni correction for multiple testing.