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. 2014 Jul;86(1):1–11. doi: 10.1124/mol.114.091595

Fig. 3.

Fig. 3.

PAR4SFT fails to dimerize with P2Y12 in saturation BRET but still dimerizes with wild-type PAR4. HEK293T cells were transiently cotransfected with (0.18 μg) of Rluc-P2Y12 (A) Rluc-PAR4 (B) and varying (amounts) of PAR4-YFP or PAR4SFT-YFP or YFP-expressing vector control to achieve YFP:Rluc ratios from 1:1 to 16:1 (0.18–2.88 μg). BRET is expressed in relative % maximal BRET. (A) PAR4SFT supported only 8.74% of PAR4 P2Y12 dimerization in saturation BRET, where PAR4YFP:RlucP2Y12 achieved an average maximum 104.40 mBU at a ratio of 16:1. (B) PAR4SFT-YFP homodimerized equally well with Rluc-PAR4, like PAR4-YFP, with an average max 268.08 mBU for PAR4YFP:RlucPAR4 and 260.24 mBU for PAR4SFTYFP:RlucPAR4 at a 16:1 ratio. Shown are the mean ± S.E.M. of 3 and 4 independent experiments, respectively. *P < 0.05; **P < 0.01.