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Chelating cytosolic calcium post PAR4 activation blocks PAR4 P2Y12 dimerization. Human platelets (4 × 108/lane) were treated with AYPGKF (150 μM) 5 minutes at 37°C with/without BAPTA-AM (20 μM) for 10 minutes at 37°C (A) or MeSAMP (100 μM) 5 minutes at room temperature (B), then immunoprecipitated with either IgG control or antibody to PAR4 (2 μg/ml). Precipitates were immunoblotted (IB) with anti-P2Y12 antibody (1:1000).
