Fig. 2.

The rate of current decline increased after desensitization with the high-affinity agonist DERM (1 µM). (A) Two experiments showing the inhibition of the DERM current at the peak of the current (left) and 10 minutes after the application of DERM (right). Example traces of the current decline induced by CNV-NLX at the peak (peak), 10 minutes after DERM (DERM, 10 minutes), and after partial blockade of MORs with β-CNA (after β-CNA) are shown. (B) Dot plot of the time constant of CNV-NLX–induced inactivation of the DERM current at the peak (peak; ●), 10 minutes after DERM (10 minutes; ○), and 5–15 minutes after slices were desensitized with ME (30 µM, 1 to 2 hours, after-ME; ○) and after treatment with β-CNA (post–β-CNA; ●). Shaded areas illustrate the standard area of the time constant of decay measured at the peak (top) and after 10 minutes (bottom). Closed squares indicate the time constant of current decay induced by CNV-NLX after the application of DERM (30 nM). Larger squares are from the current experiments, and smaller squares are from published work. Open squares are the time constant of current decay induced by CNV-NLX in slices that were predesensitized by incubation with ME (30 µM, 30–90 minutes and washed before application of CNV-NLX, after ME). *P < 0.05 by unpaired Wilcoxon–Mann–Whitney rank sum test.