TABLE 7.
Summary of all the results obtained in the case of animals from TC and VH herds that were followed up until they were slaughtered and necropsied
| Group, animal(s)a | Score in indicated analysis atb: |
|||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M0 |
M12 |
M24 |
M36 |
M48 |
Necropsy |
|||||||||||||||
| PCR | E | P | C | E | P | C | E | P | C | E | P | C | E | P | C | HP | FC | TC | PTB status | |
| Test and cull | ||||||||||||||||||||
| 1tc | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | No PTB | ||||||
| 2tc | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | No PTB | |||||||||
| 3tc to 6tc | 0 | 0 | 0 | 0 | 0 | ND | 0 | No PTB | ||||||||||||
| 7tc | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | ND | 3 | PTB |
| 8tc | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | PTB | ||||||
| 9tc | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3 | PTB | |||||||||
| 10tc | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 3 | PTB | |||||||||
| 11tc | 0 | 0 | 0 | 0 | 1 | 0 | 0 | PTB | ||||||||||||
| 12tc, 13tc | 0 | 0 | 0 | 0 | 0 | 0 | 0.7–1.3 | PTB | ||||||||||||
| 14tc, 15tc | 0 | 0 | 0 | 0 | 2–3 | 0 | 0 | 3 | 1 | 1–2 | 1–2 | 1–2 | 2.3–2.7 | PTB | ||||||
| 16tc, 17tc | 0 | 0 | 0 | 0 | 3 | 1 | 1–2 | 2 | 1–3 | 2.5–2.7 | PTB | |||||||||
| 18tc | 0 | 0 | 0 | 0 | 2 | AC | 3 | PTB | ||||||||||||
| 19tc | 1 | 0 | 0 | 0 | 2 | 1 | 1 | 3 | 1 | 1 | 2 | 2 | 2.3 | PTB | ||||||
| 20tc | 1 | 0 | 0 | 0 | 3 | 1 | 3 | 2 | 3 | 2.7 | PTB | |||||||||
| 21tc | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 2 | 3 | 3 | PTB | |||||||||
| 22tc | 1 | 0 | 1 | 0 | 3 | 0 | 0 | 2 | 1 | 2.3 | PTB | |||||||||
| 23tc | 1 | 1 | 0 | 0 | 3 | 0 | 0 | 0 | 2 | 1 | PTB | |||||||||
| 24tc | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 2 | 0 | 2 | PTB | ||||||
| 25tc to 27tc | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.3–1 | PTB | |||||||||
| 28tc, 29tc | 2 | 1–2 | 0 | 0 | 2–3 | 1 | 1–2 | 2 | 3 | 2–3 | PTB | |||||||||
| 30tc | 2 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 3 | 3 | PTB | |||||||||
| 31tc | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | PTB | |||||||||
| 32tc to 36tc | 3 | 2–3 | 0–1 | 1–2 | 2 | 1–2 | 2.7–3 | PTB | ||||||||||||
| Vaccinated | ||||||||||||||||||||
| 1vh | 0 | 0 | 0 | 0 | NC | 0 | 0 | NC | 0 | 0 | NC | 0 | 0 | 0 | 0 | 0 | No PTB | |||
| 2vh to 4vh | 0 | 0 | 0 | 0 | NC | 0 | 0 | NC | 0 | 0 | 0 | 0 | 0 | No PTB | ||||||
| 5vh, 6vh | 0 | 0 | 0 | 0 | NC | 0 | 0 | 0 | 0 | 0 | No PTB | |||||||||
| 7vh to 11vh | 0 | 0 | 0 | 0 | 0 | 0 | 0 | No PTB | ||||||||||||
| 12vh | 0 | 0 | 1 | 0 | 0 | 0 | 0 | No PTB | ||||||||||||
| 13vh | 0 | 0 | 0 | 0 | NC | 0 | 0 | NC | 0 | 0 | 0 | 0 | 0.3 | PTB | ||||||
| 14vh | 0 | 0 | 0 | 0 | NC | 0 | 0 | 1 | 0 | 0.7 | PTB | |||||||||
| 15vh | 0 | 0 | 1 | 0 | NC | 0 | 0 | 0 | 0 | 1.3 | PTB | |||||||||
| 16vh | 0 | 0 | 0 | 0 | 1 | 0 | 0 | PTB | ||||||||||||
| 17vh, 18vh | 0 | 0 | 0 | 0 | 0 | 0 | 0.7–1 | PTB | ||||||||||||
| 19vh, 20vh | 0 | 0 | 0 | 0 | 2 | 1–2 | 3 | PTB | ||||||||||||
| 21vh | 0 | 0 | 0 | 0 | NC | 0 | 0 | 2 | 3 | 3 | PTB | |||||||||
| 22vh | 1 | 2 | 0 | 0 | NC | 1 | 1 | 2 | 1 | 2.7 | PTB | |||||||||
| 23vh | 1 | 3 | 0 | 0 | NC | 0 | 0 | NC | 1 | 2 | 2 | 3 | 3 | PTB | ||||||
| 24vh | 1 | 0 | 0 | 0 | NC | 1 | 0 | NC | 0 | 0 | NC | 0 | 0 | NC | 0 | 0 | 2 | ND | 3 | PTB |
| 25vh | 1 | 2 | 0 | 0 | 0 | 0 | 0.7 | PTB | ||||||||||||
| 26vh | 1 | 0 | 0 | 0 | 1 | 0 | 0 | PTB | ||||||||||||
| 27vh | 1 | 0 | 0 | 0 | 0 | 0 | 0 | No PTB | ||||||||||||
| 28vh | 2 | 3 | 0 | 0 | NC | 1 | 1 | 2 | 2 | 3 | PTB | |||||||||
| 29vh, 30vh | 2 | 3 | 0 | 0–2 | 2 | 0–1 | 2–3 | PTB | ||||||||||||
| 31vh | 2 | 0 | 1 | 0 | NC | 0 | 0 | NC | 1 | 2 | 2 | 0 | 3 | PTB | ||||||
| 32vh | 2 | 0 | 0 | 0 | NC | 0 | 1 | 0 | 0 | 0.3 | PTB | |||||||||
| 33vh | 3 | 3 | 1 | 2 | 2 | 0 | 3 | PTB | ||||||||||||
tc, control or test and cull herds; vh, vaccinated herds.
M0 to M48, month 0 to month 48 sampling; PCR, IS900 and ISMap02 triplex real-time PCR (3, heavy shedder; 2, intermediate shedder; 1, low shedder; 0, nonshedder); E, ELISA (3, highly reactive; 2, intermediate-highly reactive; 1, intermediate or inconclusive; 0, low/nonreactive); P, conventional PCR (1, positive; 0, negative). C, culture; HP, histopathology (2, patent forms; 1, latent forms; 0, apparently free of PTB); FC or TC, fecal culture or tissue culture (3, heavy shedder/high burden; 2, intermediate shedder/burden; 1, low shedder/burden; 0 nonshedder/culture negative); ND, not done; AC, all tubes contaminated; NC, not considered because the animal(s) was vaccinated.