TABLE 1.
Oligonucleotide sequences designed for this study used in the PCR/Bioplex UPEC screening assay
| Target gene | Primer designation and sequence (5′–3′) | Concn of primer (pmol/μl) | Amplicon size (bp) |
|---|---|---|---|
| hlyA | Forward, ACGAGTTGGTTGATTAGC | 0.6 | 575 |
| Reverse, ATGCCAACAATAACCACTGCAC | 0.6 | ||
| papC | Forward, ATCAGAATACTCAAGCCAG | 0.6 | 423 |
| Reverse, TTGTTGTCTCTGTTTTGTAACGC | 0.6 | ||
| cnf1 | Forward, CACATCTGCACTCTTTGTT | 2.4 | 287 |
| Reverse, CAAATTTCCCTTCACCTG | 2.4 | ||
| uidA | Forward, TGCCGGAATCCATCGCAGCGTAAT | 1.2 | 126 |
| Reverse, ATT GGCCACCACCTGCCAGTC | 1.2 |