TABLE 3.
Performance of PCR/Bioplex technique to screen likely UPEC isolates
| Description | Primary analysis | No. of isolates | Isolation study | Measured rate (%) |
|---|---|---|---|---|
| False-negative analysis | ||||
| Nominal false negative | Microarray | 135 | Frigon et al. (29) | 17.0 |
| Bioplex underdetection | Microarray (UPEC) | 79 | Frigon et al. (29) | 3.2 |
| Overall false negative | Calculation: 17.0% + (100% − 17.0%) × 3.2% | 19.7 | ||
| False-positive analysis | ||||
| Nominal false positive | Microarray | 584 | Frigon et al. (29) | 3.9 |
| Bioplex overdetection | Calculation: (15.2% − 3.9%)/(100% − 3.9%) | 11.7 | ||
| Overall false positive | PCR/Bioplex positive | 323 | This study | 15.2a |
| PCR/Bioplex negative | 1,274 |
a
Calculated using equations 1, 2, and 3 by fitting the most likely proportion of UPECs in each sample.