FIG 5.

Neighbor-joining phylogenetic tree based on near-complete 16S rRNA gene sequences from LVBrUMA and distance-affiliated sequences. The alignment utilized fragment sizes of 1,458 nucleotide positions. The bootstrap values of 1,000 repetitions with support values above 75 are represented at nodes. The scale bar denotes the divergence percentage between sequences. LVBrUMA environmental clones are represented by gray boxes with white text. GenBank accession numbers for the nucleotide sequences are in the first set of parentheses, following the sequence name. The number of clones related to the same sequence in the clone library (0.01 distance) is in the second set of parentheses. Lake Vida brine heterotrophic strains isolated and characterized in this study are in bold (exception: LV05br1 was isolated from brine collected in 2005); the nomenclature is the same as described in the Table 1 footnote.