TABLE 1.
Summary of the heterotrophic bacterial strains isolated from Lake Vida brine collected in 2010a
| Lake Vida brine isolate(s) (GenBank ID) | No. of isolates | Medium (temp [°C]) | Closest cultivated relative (GenBank ID) | % identity (nt) | Closest-relative notes (reference) |
|---|---|---|---|---|---|
| LV10fR510-15 (KF384117) | 13 | R2A-5% NaCl (10 and 20) | Microbacterium lacus strain R-43968 (FR691402) | 98.8 (1,185/1,199) | Psychrophilic irregular rod-shaped actinobacterium isolated from Lundström Lake, Shackleton Range, Antarctica (56) |
| LV10fR510-14 (KF384118) | 1 | R2A-5% NaCl (10) | Microbacterium testaceum strain S15-M13 (AM234161) | 99.8 (1,197/1,199) | Actinobacterium isolated from radioactive waste depository Tomsk-7, Siberia, Russia (77) |
| LV10fR520-8 (KF384119) | 1 | R2A-5% NaCl (20) | Kocuria marina strain KMM 3905 (AY211385) | 99.8 (1,203/1,205) | Halotolerant denitrifier coccoid actinobacterium isolated from marine sediment from East Siberian Sea (78) |
| LV10R510-5 (KF384120); LV10R510-8 (KF384121) | 20 | R2A-5% NaCl and marine agar-5% NaCl (10 and 20) | Marinobacter sp. strain LV10S (EU908281) | 99.5 (1,211/1,217) | Psychrotolerant, halophilic rod-shaped gammaproteobacterium isolated from Lake Vida brine collected in 2005 (32) |
| LV10R510-11A (KF384122); LV10MA510-1 (KF384123) | 8 | R2A-5% NaCl and marine agar-5% NaCl (10 and 20) | Marinobacter sp. strain ELB17 (AY518678) | 99.2 (1,221/1,230) | Psychrophilic denitrifier rod-shaped gammaproteobacterium isolated from east lobe of Lake Bonney, Antarctica, at a 17-m depth (60) |
| LV10R510-2 (KF384124) | 1 | R2A-5% NaCl (10) | Marinobacter psychrophilus strain R-36953 (FR691434) | 98.6 (1,214/1,230) | Psychrophilic rod-shaped gammaproteobacterium isolated from Forlidas Pond, Pensacola Mountains, Antarctica (56) |
| LV10R520-6 (KF384125) | 3 | R2A-5% NaCl and casein agar (10 and 20) | Psychrobacter sp. strain LV414 (EU908288) | 99.5 (1,209/1,214) | Psychrophilic, halophilic coccoid gammaproteobacterium isolated from Lake Vida brine collected in 2005 (32) |
| LV10R520-3 (KF384126) | 1 | R2A-5% NaCl (20) | Arthrobacter halodurans strain JSM 078085 (EU583729) | 97.2 (1,170/1,203) | Halotolerant denitrifier actinobacterium with rod-coccus growth cycle isolated from South China Sea seawater (61) |
16S rRNA gene sequences of the isolates were compared to GenBank, and the nearest related cultivated bacterium is listed along with GenBank accession number. Isolate identifier: brine with (LV10f) and without (LV10) size fractionation. GenBank accession numbers for Lake Vida type isolates are listed. Type isolates were selected to represent the group of isolates. Nomenclature as follow: sample/medium/temperature-isolate number. Examples: LV10fR520-8 (LV10f/R5/20-8) is Lake Vida brine collected in 2010 and fractionated, isolate cultivated on R2A-5% NaCl, at 20°C, isolate number 8; LV10MA510-1 (LV10/MA5/10-1) is Lake Vida brine collected in 2010, isolate cultivated on marine agar-5% NaCl, at 10°C, isolate number 1. Isolate 16S rRNA gene sequence GenBank accession numbers are in parentheses.