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Mlc activates V. cholerae biofilm formation in LB broth only: biofilm-associated growth (A) or total growth (B) was measured for wild-type V. cholerae (WT) or a Δmlc mutant carrying either the pBAD-lacZ control vector (pCTL) or the same plasmid expressing Mlc (pmlc). Static biofilms were formed in LB broth and defined medium supplemented with glucose (Glc) or pyruvate (Pyr).
