TABLE 1.
Bacterial strains and plasmids
| Bacterial strains | Genotype and/or description | Reference or source |
|---|---|---|
| E. coli | ||
| SM10λpir | thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu λpirR6K; Kmr | 24 |
| V. cholerae | ||
| PW357 | MO10 ΔlacZ::vpsL→ lacZ; Smr | 25 |
| PW988 | PW357 ΔEIIAGlc | 20 |
| PW961 | PW357 ΔEI | 20 |
| PW1573 | PW357 ΔEI ΔEIIAGlc | This study |
| PW993 | PW357 Δmlc | 21 |
| PW1557 | PW357 ΔEI Δmlc | This study |
| PW1558 | PW357 ΔEI ΔEIIBCGlc | This study |
| PW1559 | PW357 ΔEl ΔEIIBCGlc Δmlc | This study |
| PW996 | PW357 ΔEIIBCGlc Δmlc | 21 |
| Plasmids | ||
| pBAD-TOPO | Arabinose-inducible expression vector used to generate His6/V5 epitope-tagged proteins | Life Technologies |
| pBAD-TOPO-lacZ | pBAD-TOPO containing the lacZ gene; provided as a control | Life Technologies |
| pBAD-TOPO-EIIAGlc rev | pBAD-TOPO containing the EIIAGlc gene in reverse, noncoding orientation | 20 |
| pBAD-TOPO- MlcV. cholerae | pBAD-TOPO containing full-length V. cholerae mlc::His6/V5 | This study |
| pBAD-TOPO-MlcE. coli | pBAD-TOPO containing full-length E. coli mlc::His6/V5 | This study |