FIG 5.

Determination of the relative DNA content of wild-type (GCB908) versus resT-inducible spirochetes (GCB2127) by quantification of fluorescence intensity of Hoechst 33258-stained spirochetes in fluorescence micrographs. Images of fluorescent spirochetes were analyzed for total fluorescence intensity by collecting the integrated fluorescence density using ImageJ (NIH) and correcting for the fluorescence background. The mean fluorescence intensity ± the standard error is plotted. Statistical significance was determined by a two-tailed unpaired t test and is indicated above the graphs (*, P < 0.05; **, P < 0.01; n.s., not significant).