FIG 7.

Southern blot of genomic DNA from a resT-inducible strain (GCB2127). Spirochetes were grown either with or without IPTG, and the culture was sampled at 0, 24, 48, 72, and 96 h. DNA was extracted and run on a 0.65% agarose field inversion gel and transferred to a nylon membrane for Southern blotting. Probes were generated against the left end of lp17 (A) as well as the right end of lp28-2 (B) as described in Materials and Methods, using PCR primers B2275 and B2276 for lp17 and B2295 and B2296 for lp28-2.