FIG 8.

Treatment of DNA from the resT-inducible strain (GCB2127) and the wild-type parent (GCB908) with the telomere resolvase ResT. (A) Schematic of the experimental design. To simplify detection of telomere ends, DNA was digested with a restriction enzyme to liberate either a free telomere or the replicated telomere junction. ResT activity on the replicated junction generates free ends, which were detected by Southern blotting hybridization. (B and C) Southern blots of DNA that was isolated 72 h after IPTG washout and digested with EcoRI or BamHI prior to being treated with ResT. DNA was run on a 1% agarose gel and transferred to a nylon membrane for Southern blotting hybridization with probes for the lp17 (B) and lp28-2 (C) left ends.