FIG 7.

The G1041A mutation results in the expression of a new transcript upstream from pqsD. The P. aeruginosa wild-type strain PAO1 containing a reporter plasmid with either the wild-type sequence (ppqsDwtTcZ, indicated as “1wt”) or nested deletion plasmids with the pqsC point mutation (ppqsDG1041ATcZ 1 through 6) was grown in VBG until an OD₆₆₀ of 0.2 was reached. The cultures were then assayed for β-Gal activity, which is presented in Miller units as the mean ± σ^{n − 1} of results from at least three separate experiments. The numbers on the fusion diagram indicate the distance of the 5′ end of each fusion from the pqsD start codon. The asterisk indicates the location of the G1041A mutation.