FIG 2.

Overexpression of flgA, flgH, and flgI suppresses the motility defect of the flgG* G53R filamentous rod mutant. The genes required for PL-ring formation (flgA, flgH, and flgI) were cloned into the arabinose-inducible ΔaraBAD locus in every filamentous rod mutant isolated to date. The chromosomal copies of flgA, flgH, and flgI were left intact in the flg operon. The motility defect of the G53R mutant was suppressed to nearly WT levels when inoculated in soft agar with 0.2% arabinose added. WT, DaraBAD::flgAHI.