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FlgJ is secreted extracellularly. Fifteen ml of culture from TH17693 (flgJ8013::HA) was collected at each OD600 unit from 0.4 to 1.1. Cells were removed, and the supernatant protein was concentrated and analyzed by SDS-PAGE using anti-HA antibody. Anti-DnaK antibody was added as a cell lysis control.
