FIG 3.

Effect of fliA (σ²⁸) alleles on levels of secreted FlgM and cell motility. (A) Levels of secreted FlgM were determined for strains carrying different alleles of the σ²⁸ structural gene fliA by quantitative Western blot analysis using anti-FlgM antibody to detect FlgM in the supernatant of the spent growth medium (Sec FlgM, secreted FlgM; WC FlgM, whole cellular FlgM; WC FliA, whole cellular FliA; WC DnaK, whole cellular DnaK). P*, parent strain, TH18500 (ΔaraBAD1156::flgM⁺). (B) Relative levels of secreted FlgM. (C) Motility assay with various mutant backgrounds, depicted with the ΔaraBAD1156::flgM⁺ strain (WT), in the absence and presence of added 0.2% arabinose.