FIG 4.
Effect of flagellar late substrate deletion, growth phase, and fljBenx vh2 mutations on levels of secreted FlgM. (A) Secreted levels of FlgM were determined for strains carrying different flagellum late substrate gene (flgK, flgL, fliC, fliD, and fljB) deletion mutants, flagellum-specific phase (Δhin-5717 and Δhin-5718) mutants, and fljBenx vh2 mutants by quantitative Western blot analysis using anti-FlgM antibody to detected FlgM in the supernatant of the spent growth medium. Sec FlgM, secreted FlgM; Sol FlgM, cellular soluble FlgM; Insol FlgM, cellular insoluble FlgM; WC DnaK, whole cellular DnaK. P*, parent strain, TH18500 (ΔaraBAD1156::flgM+). (B) Relative secreted FlgM levels.