FIG 8.

Effects of NaCl and KCl ionic strengths on levels of secreted FlgM and cell motility. (A) Secreted levels of FlgM were determined with strain P*, the parent strain, TH18500 (ΔaraBAD1156::flgM⁺), in LB-0.2% arabinose medium with NaCl and KCl added at concentrations depicted by quantitative Western blot analysis, using anti-FlgM antibody to detect FlgM in the supernatant of the spent growth medium. Sec FlgM, secreted FlgM; Sol FlgM, cellular soluble FlgM; Insol FlgM, cellular insoluble FlgM; WC DnaK, whole cellular DnaK. (B) Relative levels of secreted FlgM. (C) Motility assay using various concentrations of NaCl and KCl, depicted with strain TH18500, in the absence and presence of added 0.2% arabinose (Ara).