FIG 4.
Txc-dependent secretion of CbpE by strain PA7 overproducing TtsSc or TtsRc. (a and b) Immunoblots of extracellular protein fractions from wild-type PA7 (PA7) or Txc-deficient PA7 (PA7 Δtxc) carrying the empty vector (pBBR), pTtsSc (a), or pTtsRc (b), probed with anti-CbpE antibody. (c) Immunoblots of intracellular (top) and extracellular (bottom) fractions from wild-type PA7 (PA7) and the Txc-deficient PA7 strain (PA7 Δtxc) carrying pTtsSc alone or pTtsSc and pRGP69, probed with anti-CbpE antibody. CbpE and degradation products of CbpE are indicated by asterisks. Molecular mass markers (in kDa) are indicated on the left.