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(A) GldA activity in Δpfl mutant cells cultured in M-MRS broth (●) or M-MRS broth containing 100 mM glycerol (○). Data are the means of results from three experiments. Bars indicate standard errors. (B) RT-PCR to detect glpF1 and glpF2 transcripts in Δpfl mutant cells. The Δpfl mutant was cultured in M-MRS broth containing 100 mM glycerol under anaerobic conditions. DNA size markers (M) are shown in kb.
