TABLE 2.

Primers used in this study

Category and primer name	Sequence (5′ to 3′)a
Construction of suicide plasmid for pflB gene
EF delta pfl XbaI F	GCGTCTAGACGCTTGCATCTAAAAGGTTATGTG
EF delta pfl HindIII R	CTCCAAGCTTGGAGGCTCAATTCCTTCTAATGG
pHY300PLK tet P (+) SalI	FCCGGTCGACCGGGCCATATTGTTGTATAAGTG
pHY300PLK tet R	GCTTCTAGAGATCTGCAGGTCG
Confirmation of pflB gene disruption
EF delta pfl check F	GGGCACACAAGAAGTTATTCAGTC
EF delta pfl check R	GGCATATTGAGTTTCTTCACTTGG
Construction of reporter assay and plasmids
EF ldhL1 Pro XbaI F	AAGTCTAGACTTTCATTCTCCTTGGATTATAAGC
EF ldhL1 Pro SalI R	ATGGTCGACCATGTGTACCATTCCTTCCTCTAC
EF pdhAB Pro XbaI F	TGCTCTAGAGCAATGAATGTTCGTGATAAAACC
EF pdhAB Pro SalI R	ATGGTCGACCATTTTGTCACACAATCCTCTCTATTACG
EF pflB Pro XbaI F	CCCTCTAGAGGGCACACAAGAAGTTATTCAGTC
EF pflB Pro SalI R	ATGGTCGACCATGAAGTGTTTGCCTCCTTAGTT
EF adhE Pro F	GGTTAAGCCGAATGAATTTTTTGAC
EF adhE Pro SalI R	ATGGTCGACCATGTTCATCCTCCTAAGAGTTTCATT
beta-gal SalI F	GACGTCGACGTCGTTTTACAACGTCGTGACTG
beta-gal SD ATG-fuse XbaI F	GTTTCTAGAAACTAAGGAGGCAAACACTTCATG
beta-gal SphI R	AGGGCATGCCCTGCCCGGTTATTATTATTTTTGA
RT-PCR
EF RT-PCR 16S F	GTGTCGTTGATGGATGGACC
EF RT-PCR 16S R	TCTACGCATTTCACCGCTACAC
EF RT-PCR pflA F	CCGTTTTATCGTATTTACACAAGGG
EF RT-PCR pflA R	GGCATCCAGACGGATTAAATATTC
EF RT-PCR pflB F	GAGAACAAGCGTTGACACTCG
EF RT-PCR pflB R	CCATATAATGCGATACGACGATAGTC
EF RT-PCR glpF1 F	CGAACAAAATACGCTGGTTCTG
EF RT-PCR glpF1 R	CAAACAAGCAAGCCAACAGC
EF RT-PCR glpF2 F	GGTTGGGTCGTTATTGCTTTAGG
EF RT-PCR glpF2 R	ATTGGCTGGATAGTTACGGACAG

^aUnderlined nucleotides indicate restriction sites.