FIG 3.

Germination of PS533 spores (wild type) made in rich and poor liquid media and with and without heat activation. (A and B) Germination of ∼400 heat-activated (A) and ∼400 unactivated (B) individual spores made in rich medium with 0.33 (▼), 1.0 (▲), 3.3 (•), or 10 (◼) mM l-valine for 5.0 min, followed by blocking further commitment by method B, was monitored as described in Materials and Methods. (C) Germination of ∼500 heat-activated individual spores made in the poor medium with 1.0 (▲), 3.3 (•), or 10 (◼) mM l-valine for 40 min, followed by blocking further commitment by method B, was monitored as described in Materials and Methods. Vertical arrows denote the times of germinant removal and its replacement with d-alanine.