FIG 5.
Percentages of Trelease times of PS533 spores (wild type) as a function of germination time with either continuous l-valine exposure or two short l-valine exposures. Spores were germinated with 10 mM l-valine for 90 min with no germinant removal (A) or for 2.5 (B), 5.0 (C), and 10 (D) min followed by germinant removal by method A, further incubation until 45 min, a second 2.5-min exposure to 10 mM l-valine, germinant removal again by method A, and further incubation until 90 min, although there were no changes after 80 min. Germination of ∼400 individual spores was followed as described for panels A to D, and the percentages of spores with Trelease values in 3.0-min windows were summed and plotted against the germination time.