FIG 6.
Germination of PS3415 spores (high GerB* GR levels) with continuous l-asparagine exposure or with two short l-asparagine exposures. (A) Spores were germinated with 0.5 mM l-asparagine either continuously (◼) or for two 1.0-min exposures separated by 45 min (•), with each exposure followed by removal of l-asparagine by method A using a pipette for germinant removal and rinsing and continued incubation in buffer as described in Materials and Methods. (B) Distribution of Trelease values of spores given two 1.0-min exposures to 0.5 mM l-asparagine, with l-asparagine removed after each exposure as described above and further incubation as described in Materials and Methods. Trelease values were summed over 1-min windows in germination. (C) DIC image intensities of 10 individual PS3415 spores that became committed during the separate 1.0-min exposures to 0.5 mM l-asparagine. (D) DIC image intensities of five individual PS533 spores that had not become committed during the 1-min exposures to 0.5 mM l-asparagine.