FIG 5.

Long-term growth of 1457 ΔADI results in oxidative stress conditions and subsequent cell death. (A) Strain 1457 and 1457 ΔADI cells growing in TSB supplemented with 35 mM glucose and 5 mM arginine with or without buffering by MOPS were removed at 24 and 72 h postinoculation, doubly labeled with CTC and HPF, and measured by flow cytometry. (B) Cells were also removed at 72 h for analysis by electron paramagnetic resonance spectroscopy (EPR). (C) Survival of cultures was monitored daily for 5 days by cell enumeration.