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Analysis of maturing flow cell biofilms. (A) Total RNA was extracted from cells growing in maturing biofilms and used for Northern blot analysis of arcA1 and arcA2. (B and C) Spent medium effluent from a maturing flow cell biofilm was collected and subjected to analysis for products of arginine deiminase activity (B) and glucose metabolism (C). (D) Biofilm-grown cells were removed at 72 h postinoculation and subjected to paramagnetic resonance spectroscopy (EPR) analysis.
