TABLE 2.
Primers used in the studya
| Product | Forward primer | Reverse primer |
|---|---|---|
| arcA1B1D1R1 | 2009 CCGGGCATGCAAGACGACACCAGGATTTGC | 2010 CCGGGGATCCATTATGTATGAGTGATACGC |
| arcA2D2R2B2C2 | 2011 CCGGGCATGCACATCTCCTGGCTTTGCTCA | 2012 CCGGGGATCCAATAGCCTAGAACATGGTG |
| Upstream and 5′ region of arcA1 | 1068 ATCCTAGGATCCGCAGATATTATTGATTAC | 1069 ATCCTATCTAGACGTCATCTATAAATTGC |
| Downstream and 3′ region of arcA1 | 1070 ATCCTAGTCGACCGTCCCGGTGTGGTTGT | 1071 ATCCTACTGCAGCGGCAGTATGAATGTTATC |
| Upstream and 5′ region of arcA2 | 1074 ATCCTAGGATCCGGCAAGACATTTTGGAG | 1075 ATCCTATCTAGACTCGCGTACTTCTGGCTC |
| Downstream and 3′ region of arcA2 | 1076 ATCCTAGTCGACGCTATTGGTATATCAGAACG | 1077 ATCCTACTGCAGCTTGGAAGTGTGTTACCTCC |
| tetM tetracycline resistance cassette | 1638 ATCCTAGGATCCGGTACATGATTACAGATAC | 1639 ATCCTAGGATCCCGATCTCCTCCTTTCCAC |
| dhfr trimethoprim resistance cassette | 887 GGCATAGGATCCCGATTGTCAGGCTTAATGG | 888 GGCATAGGATCCGCAACTTAGGGAATGTTTATGG |
| arcA1 DNA probe | 1130 GTACGCAAAGAAGAAATACAACTTG | 1129 TGCTGAATGTGTAGTGAATTTGTCG |
| arcA2 DNA probe | 1682 CTTAGATCCAATGCCCAACC | 1683 CATACATCTTGGGCCTCCGC |
a
Underlined portions of the primers correspond to restriction enzyme recognition sites. GCATGC, SphI; GGATCC, BamHI; TCTAGA, XbaI; GTCGAC, SalI; CTGCAG, PstI; GCTAGC, NheI.