FIG 1.
Cluster of l-arabinose utilization genes on the chromosome of C. glutamicum ATCC 31831. (A) The locations and directions of transcription of the six genes (araA, araB, araD, araE, araR, and galM), based on a previous study (26), are shown, and the fragments used as DNA probes (PA, PB, PC, and PD) in electrophoretic mobility shift assays are indicated by solid lines. The nucleotide sequences of the araB-galM intergenic region (B) and the araE upstream region (C) are shown. The translation start codons are boxed, and the putative −10 and −35 regions, based on the transcription start sites (+1) determined, are indicated by asterisks above the sequences. The regions protected by AraR in DNase I footprinting analysis are indicated in capital letters against a gray background.