FIG 10.

Effects of disruption of l-arabinose catabolic genes on l-arabinose-inducible gene expression. The C. glutamicum ATCC 31831 wild-type (white bars), araA (gray bars), araB (dark gray bars), and araD (black bars) deletion mutant strains were grown in nutrient-rich A medium for 4 h, and exponentially growing cells were then supplemented with l-arabinose at a final concentration of 2% (wt/vol). The levels of araB and araA (A), araE (B), and araR (C) in cells incubated with l-arabinose for 0, 5, and 15 min were determined by qRT-PCR. The mRNA levels are presented relative to the values obtained for wild-type cells incubated with l-arabinose for 5 min. Mean values and standard deviations for at least three independent cultures are shown.