FIG 11.

Effects of araE disruption on l-arabinose-dependent upregulation of araB (A), araE (B), and araR (C). The C. glutamicum ATCC 31831 wild-type (WT) and araE deletion mutant (ΔaraE) strains were grown in nutrient-rich A medium for 4 h, and exponentially growing cells were then supplemented with l-arabinose to final concentrations of 0.5 mM (white bars), 5 mM (light gray bars), 50 mM (dark gray bars), and 200 mM (black bars). The mRNA levels in the cells incubated with l-arabinose for 0 and 5 min were determined by qRT-PCR and are presented relative to the values obtained for wild-type cells incubated with 50 mM l-arabinose. Mean values and standard deviations for at least three independent cultures are shown.