FIG 4.

Effects of araR disruption on expression of araB (A), araE (B), and galM (C). The C. glutamicum ATCC 31831 wild-type strain (WT) and the araR deletion mutant strain (ΔaraR) were grown in nutrient-rich A medium for 4 h, and exponentially growing cells were then supplemented with l-arabinose to a final concentration of 2% (wt/vol). The mRNA levels in cells incubated with l-arabinose for 0 min (−) or 5 min (+) were determined by qRT-PCR and are presented relative to the values obtained for the wild-type cells before addition of l-arabinose. Mean values and standard deviations for at least three independent cultures are shown.