FIG 5.
In vitro binding of AraR to the upstream regions of the l-arabinose utilization genes. EMSA was carried out with the AraR protein. The DNA probes used were the upstream regions of araB (PA) (A), galM (PB) (B), araE (PC) (C), and araR (PD) (D) shown in Fig. 1A. Each 20-μl binding reaction mixture, containing the DNA probe at 1 nM and the AraR protein at various concentrations (lanes 1, 0 nM; lanes 2, 10 nM; lanes 3, 25 nM; lanes 4, 50 nM; lanes 5, 75 nM; lanes 6, 100 nM; lanes 7, 125 nM; lanes 8, 150 nM; lanes 9, 175 nM; and lanes 10, 200 nM), was subjected to electrophoresis on a 6% polyacrylamide gel.