FIG 7.
Effects of l-arabinose and d-xylose on the DNA-binding activity of AraR. EMSA was carried out with the AraR protein and the araB upstream region. Each 20-μl binding reaction mixture, containing the DNA probe PA (Fig. 5) at 1 nM with or without the AraR protein at 100 nM, was subjected to electrophoresis on a 6% polyacrylamide gel. The reaction mixture was supplemented with l-arabinose or d-xylose at various concentrations (1, 5, and 15 mM). The free DNA and the DNA-protein complex are indicated by white and black arrowheads, respectively.