TABLE 1.
Strains used in this study
| Strain | Genotype and description | Reference or source |
|---|---|---|
| E. coli | ||
| EC448 | MC4100 Δ(λattL-lom)::bla lacIq P204::ftsZ-gfp | 62 |
| Omnimax-2 T1R | F′ [proAB+ lacIq lacZΔM15 Tn10(TetR) Δ(ccdAB)] mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD | Invitrogen |
| HB101/pRK24 | F− mcrB mrr hsdS20(rB− mB−) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 | 20 |
| RAN334 | Omnimax/pRAN334 (Ptet::CFPopt cat) | This study |
| RAN357 | Omnimax/pRAN357 (Ptet::CFPopt-MCS cat) | This study |
| RAN410 | Omnimax/pRAN410 (Ptet::CFPopt-mldA cat) | This study |
| RAN460 | Omnimax/pRAN460 (Ptet::CFPopt-mldB cat) | This study |
| RAN461 | Omnimax/pRAN461 (Ptet::CFPopt-mldC cat) | This study |
| C. difficile | ||
| JIR8094 | Spontaneous erythromycin-sensitive derivative of C. difficile strain 630 | 13 |
| RAN125 | JIR8094 mldA248::ItrB::ermB | This study |
| RAN258 | JIR8094 mldB153::ItrB::ermB | This study |
| RAN427 | JIR8094/pRAN414 (Ptet::mldABC cat) | This study |
| RAN428 | JIR8094/pRAN415 (Ptet::mldAB cat) | This study |
| RAN429 | JIR8094/pRAN416 (Ptet::mldA cat) | This study |
| RAN430 | JIR8094/pRAN417 (Ptet::mldBC cat) | This study |
| RAN431 | JIR8094/pRAN418 (Ptet::mldB cat) | This study |
| RAN432 | JIR8094/pRAN419 (Ptet::mldC cat) | This study |
| RAN433 | JIR8094/pRPF185 (Ptet::gusA cat) | This study |
| RAN446 | RAN125 (mldA::ItrB::ermB)/pRAN414 (Ptet::mldABC cat) | This study |
| RAN447 | RAN125 (mldA::ItrB::ermB)/pRAN415 (Ptet::mldAB cat) | This study |
| RAN448 | RAN125 (mldA::ItrB::ermB)/pRAN416 (Ptet::mldA cat) | This study |
| RAN449 | RAN125 (mldA::ItrB::ermB)/pRAN417 (Ptet::mldBC cat) | This study |
| RAN450 | RAN125 (mldA::ItrB::ermB)/pRAN418 (Ptet::mldB cat) | This study |
| RAN451 | RAN125 (mldA::ItrB::ermB)/pRAN419 (Ptet::mldC cat) | This study |
| RAN452 | RAN125 (mldA::ItrB::ermB)/pRPF185 (Ptet::gusA cat) | This study |
| RAN453 | RAN258 (mldB::ItrB::ermB)/pRAN414 (Ptet::mldABC cat) | This study |
| RAN454 | RAN258 (mldB::ItrB::ermB)/pRAN415 (Ptet::mldAB cat) | This study |
| RAN455 | RAN258 (mldB::ItrB::ermB)/pRAN416 (Ptet::mldA cat) | This study |
| RAN456 | RAN258 (mldB::ItrB::ermB)/pRAN417 (Ptet::mldBC cat) | This study |
| RAN457 | RAN258 (mldB::ItrB::ermB)/pRAN418 (Ptet::mldB cat) | This study |
| RAN458 | RAN258 (mldB::ItrB::ermB)/pRAN419 (Ptet::mldC cat) | This study |
| RAN459 | RAN258 (mldB::ItrB::ermB)/pRPF185 (Ptet::gusA cat) | This study |
| RAN346 | JIR8094/pRAN334 (Ptet::CFPopt cat) | This study |
| RAN424 | JIR8094/pRAN410 (Ptet::CFPopt-mldA cat) | This study |
| RAN462 | JIR8094/pRAN460 (Ptet::CFPopt-mldB cat) | This study |
| RAN463 | JIR8094/pRAN461 (Ptet::CFPopt-mldC cat) | This study |
| RAN465 | RAN125 (mldA::ItrB::ermB)/pRAN410 (Ptet::CFPopt-mldA cat) | This study |
| RAN466 | RAN125 (mldA::ItrB::ermB)/pRAN460 (Ptet::CFPopt-mldB cat) | This study |
| RAN467 | RAN125 (mldA::ItrB::ermB)/pRAN461 (Ptet::CFPopt-mldC cat) | This study |
| RAN469 | RAN258 (mldB::ItrB::ermB)/pRAN410 (Ptet::CFPopt-mldA cat) | This study |
| RAN470 | RAN258 (mldB::ItrB::ermB)/pRAN460 (Ptet::CFPopt-mldB cat) | This study |
| RAN471 | RAN258 (mldB::ItrB::ermB)/pRAN461 (Ptet::CFPopt-mldC cat) | This study |