TABLE 2.
Plasmids used in this study
| Plasmid | Relevant feature(s) | Reference or source |
|---|---|---|
| pRPF185 | E. coli-C. difficile shuttle vector with tetracycline-inducible promoter; Ptet::gusA cat CD6ori RP4oriT-traJ pMB1ori | 21 |
| pCE240 | E. coli-C. difficile shuttle vector for creating C. difficile mutants using TargeTron mutagenesis; ltrB::ermB::RAM ltrA cat pIP404ori pMB1ori RP4oriT | 18 |
| pMC123 | E. coli-C. difficile shuttle vector; cat bla CD6ori RP4oriT pMB1ori | 17 |
| pTHE1037 | E. coli-C. difficile shuttle vector; cat CD6ori RP4oriT pMB1ori | This study |
| pBL100 | E. coli-C. difficile shuttle vector for creating C. difficile mutants using TargeTron mutagenesis; ltrB::ermB::RAM ltrA cat bla CD6ori RP4oriT pMBlori | 19 |
| pSW4-CFPopt | Template for PCR of CFPopt | 22 |
| pDSW1215 | pCD240/mldA248 | This study |
| pRAN101 | pTHE1037/mldA248 ltrB::ermB::RAM ltrA | This study |
| pRAN243 | pBL100/mldB153 | This study |
| pRAN414 | pRPF185 Ptet::mldABC | This study |
| pRAN415 | pRPF185 Ptet::mldAB | This study |
| pRAN416 | pRPF185 Ptet::mldA | This study |
| pRAN417 | pRPF185 Ptet::mldBC | This study |
| pRAN418 | pRPF185 Ptet::mldB | This study |
| pRAN419 | pRPF185 Ptet::mldC | This study |
| pRAN334 | pRPF185 Ptet::CFPopt | This study |
| pRAN357 | pRPF185 Ptet::CFPopt-MCS | This study |
| pRAN410 | pRPF185 Ptet::CFPopt-mldA | This study |
| pRAN460 | pRPF185 Ptet::CFPopt-mldB | This study |
| pRAN461 | pRPF185 Ptet::CFPopt-mldC | This study |