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. 2014 Jun;196(12):2143–2154. doi: 10.1128/JB.01484-14

FIG 2.

FIG 2

Tetrameric LeuO wt binds to DNA with a high affinity. The LeuO wt (1.5 μg per lane) purified under native (LeuO6×His-NC) and denaturing (LeuO-6×His-DC) conditions was analyzed in a native 10% polyacrylamide gel (A), in a Western blot after SDS-PAGE with anti-His antibody (B), and by EMSA with different concentrations of LeuO6×His-NC (tetramer) (D) and LeuO-6×His-DC (monomer > dimer > trimer > tetramer) (E). (C) Schematic representation of the F+1 DNA fragment from the ompS1 5′ regulatory region (13) used as a probe for EMSAs.