TABLE 1.
Real-time RT-PCR primers used in this study
| Primer | Mu50 ORFa | Sequence |
|---|---|---|
| 16SrRNA RTF | SAVrRNA16 | CGTGCTACAATGGACAATACAAA |
| 16SrRNA RTF | SAVrRNA16 | ATCTACGATTACTAGCGATTCCA |
| citB RTF | SAV1350 | GCGCAACAGCAACTGATTTA |
| citB RTR | SAV1350 | GTTGTACACCTGGACCAAAGA |
| feoB RTF | SAV2584 | GGAATGACAGCAACACAGTTAC |
| feoB RTR | SAV2584 | GTGCTGACTGACCACCTAAA |
| sbnA RTF | SAV0116 | TTCTGTAGGGCAAACACCTATG |
| sbnA RTR | SAV0116 | GCTGCCTCCAGGATTCATATAC |
| sstC RTF | SAV0735 | GACCTAATGGTGCGGGTAAG |
| sstC RTR | SAV0735 | CAGACATGAGCTGTCCATCTATT |
a
Gene designations based on the Staphylococcus aureus strain Mu50 genomic DNA sequence (68). ORF, open reading frame.