FIG 3.

Identification of the ΔtetH mutant of A. ferrooxidans ATCC 23270 by Southern blotting. (A) Schematic chart of the genomic region bearing the tetH gene and showing the position of the probe and the sites of EcoRV. The sizes between the two EcoRV sites are indicated on the right. (B) Southern blot analyses of EcoRV-digested genomic DNA from the wild type (WT) and the ΔtetH mutant (MT) of A. ferrooxidans ATCC 23270. Lane M represents the DNA molecular markers, and the sizes of the fragments are indicated on the left.