TABLE 2.
Plasmids used in this study
| Plasmid | Characteristics | Resistance | Reference or source |
|---|---|---|---|
| pASK-IBA2 | AHTC-inducible expression vector containing ompA signal sequence | Ampr | IBA GmbH |
| pASK-IBA33plus | AHTC-inducible expression vector without ompA signal sequence | Ampr | IBA GmbH |
| pPK3 | pCR-Blunt II TOPO with 3.1-kb BadA HN23 insert | Kmr | 15 |
| pUC-A1 | pUC13 containing yadA gene | Ampr | 20 |
| pTS1 | pASK-IBA33plus containing yadA membrane anchor sequence | Ampr | This study |
| pTS2 (hybrid 1) | pASK-IBA2 containing ompA signal sequence-badA HN23-badA membrane anchor construct | Ampr | This study |
| pTS3 (hybrid 2) | pASK-IBA33plus containing badA signal sequence-badA HN23-yadA membrane anchor construct | Ampr | This study |
| pTS4 | pASK-IBA33plus containing BadA HN23 | Ampr | This study |
| pTS5 | pASK-IBA2 containing yadA membrane anchor sequence | Ampr | This study |
| pPK5 (hybrid 3) | pASK-IBA2 containing ompA signal sequence-badA HN23-yadA membrane anchor construct | Ampr | This study |