FIG 2.
ΔflgN strains are nonmotile. Swim expansion assays (A and B) and swarm expansion assays (C and D) were performed. Wild-type (3610; filled circle), Δhag (DS1677; filled triangle), and ΔflgN (NRS3570; open circle) strains were used for the experiments shown in panels A and C. Wild-type (3610; filled circle) and ΔflgN (NRS3570; open circle) strains along with the ΔflgN amyE::Phy-spank-flgN-lacI (NRS3578) strain without (filled triangle) and with (open triangle) 10 μM IPTG induction were used for the experiments shown in panels B and D. Each graph is representative of three independent biological replicates. (E) Photographs of swarm expansion plates taken at the end of the assay, after 6 h of incubation at 37°C. (F) RT-PCR analysis of transcription of rRNA, flgN, flgK, and flgL in wild-type (NCIB3610), ΔflgN (NRS3570), and ΔflgK-flgL (NRS4060) strains. Genomic DNA (gDNA) and H2O are shown as positive and negative controls for amplification, respectively. Reaction mixtures were incubated with (+) or without (−) reverse transcriptase (RT).