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. 2014 Jun;196(12):2216–2226. doi: 10.1128/JB.01599-14

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Copyright © 2014 Cairns et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

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FIG 3 — Deletion of flgN results in a loss of bimodal hag transcription. (A and B) Flow cytometry analysis of hag transcription in strains carrying the P_hag-yfp transcriptional reporter fusion. Strain 3610 was used as a nonfluorescent control. Shown are the wild-type (WT; NRS3076), ΔflgN (NRS3570), and ΔflgN amyE::P_hy-spank-flgN-lacI (NRS3713) strains without and with induction with 50 μM IPTG. (C) Fluorescence microscopy analysis of the wild-type (NRS3076) and ΔflgN (NRS3708) strains carrying the P_hag-yfp transcriptional reporter (false-colored green). Scale bar, 5 μm. Asterisks indicate examples of cells that do not transcribe hag. Max, maximum; AU, arbitrary units.