TABLE 1.
qPCR and culture examinations of stem and leaf samples from in vitro-grown bean plantsa
| Plant part and no. or parameter | No. of cells/gb | No. of CFU/g after cultivation: |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Without decontamination |
With decontamination |
||||||||
| MB + PANTAc | MBd | HEYMe | Leslief | MB + PANTA | MB | HEYM | Leslie | ||
| Stem | |||||||||
| 1 | 6.31 × 106 | >1,000 | >1,000 | 100 | 0 | 150 | 0 | 0 | 0 |
| 2 | 1.54 × 107 | 100 | 0 | 0 | 200 | 20 | 0 | 0 | 0 |
| 3 | 7.74 × 106 | 1,000 | >200 | >1,000 | 200 | 10 | 10 | 0 | 0 |
| Mean (SD) | 9.81 × 106 (4.88 × 106) | 3,700 (5,474) | 3,400 (5,716) | 366 (550.7) | 133.33 (115.5) | 60 (78.1) | 3.33 (5.7) | 0 (0) | 0 (0) |
| Leaf | |||||||||
| 1 | 6.70 × 106 | 200 | >200 | 1,000 | 0 | 0 | 100 | 100 | 10 |
| 2 | 1.74 × 106 | >200 | >200 | 500 | 100 | 0 | 0 | 0 | 0 |
| 3 | 3.49 × 106 | >100 | >200 | >1,000 | 0 | 0 | 100 | 10 | 100 |
| Mean (SD) | 3.97 × 106 (2.51 × 106) | 166 (57.73) | 200 (0) | 3,833 (5,346) | 33.33 (57.7) | 0 (0) | 66.67 (57.7) | 36.67 (55) | 36.67 (55) |
| Negative control | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
a
The input concentration of M. avium subsp. hominissuis culture added to the agar was 109 cells/ml.
b
Determined by qPCR assay for IS1245.
c
MB + PANTA, Middlebrook M7H11 plus PANTA.
d
MB, Middlebrook M7H11 without antibiotics.
e
HEYM, Herrold egg yolk medium.
f
Leslie, Leslie medium.