FIG 1.

(a) Phylogenetic placement of BiG as a singleton clade among five orders of Gammaproteobacteria. Maximum-likelihood reconstruction inferred from 89 concatenated SiCO genes (27,452 aligned amino acid sites). (b) Location of BiG among members of the insect gut-associated Orbales. The tree is based on maximum likelihood with the 16S rRNA gene. (c) Proportions of the 89 individual SiCO gene trees that returned each phylogenetic pattern with their average (Avg) bootstrap support at the indicated node and average gene length. (d) Proportions of individual SiCO gene trees that united the BiG and the Apis mellifera Gamma bin (Gbin) gene copies as a monophyletic clade and average bootstrap support. Asterisks represent bootstrap support values of 100, and values below 50 are not shown. Str, strain; SS, secondary symbiont.