TABLE 2.
Recovery of DNA in a 5-kb ladder recovered after filtration through AAO (0.02-μm Anotop 25) filters
Sample | % recoverya |
---|---|
Filtrate | 64 (12) |
Wash 1 | 10 (2) |
Wash 2 | 2 (0.5) |
Wash 3 | 0.6 (0.3) |
Extract | 7 (2) |
Total | 84 (12) |
Percentages of DNA in the filtrate and washes represent material passing through the filters, and that in the extract represents material recovered from the filters using a back-flush protocol. Values are means (standard deviations) from triplicate assays.