TABLE 3.

Recovery of nucleic acids from microorganisms collected on AAO (0.02-μm Anotop 25) filters

Controla	Sampleb	ID	Extraction methodc	Variable	% recoveryd
					DNA	RNA
Liquid	Phage	A	MP	Flush direction	73 (10)
	Bacteria	B	MP	Flush direction	96 (7)
		I	MP	Chemistry, 1	84 (8)	73 (5)
		J	MP	Chemistry, 2	102 (4)	91 (4)
	Protist	H	MP	Vol, 1 ml	89 (12)
		H	MP	Vol, 2 ml	82 (14)
		H	MP	Vol, 4 ml	72 (11)
	Seawater, KB	D	MP	% recovery	117 (23)
		G	MP	Vol, 10 ml	130 (52)
		G	MP	Volume 50 ml	68 (53)
		G	MP	Vol, 250 ml	93 (30)
		G	MP	Vol, 500 ml	113 (36)
		G	MP	Vol, 1 liter	95 (33)
All liquid					93 (24)	82 (12)
Pelleted*	Protist	C	MP	Flush direction	144 (12)
	Seawater, AW	K	MP, 60	Chemistry + Lys	91 (11)
		K	MP, 60 + Lys	Chemistry + Lys	92 (14)
		K	LB1, 60	Chemistry + Lys	110 (38)
		K	LB1, 60 + Lys	Chemistry + Lys	65 (19)
Pelleted**	Seawater, KB	E	MP	% recovery	112 (7)	134 (15)
		F	MP	Vol,10 ml	132 (33)
		F	MP	Vol, 50 ml	69 (49)
		F	MP	Vol, 250 ml	95 (6)
		F	MP	Vol, 500 ml	115 (7)
		F	MP	Vol, 1 liter	97 (15)
All pelleted					102 (19)	134 (15)
Pelleted** + supernatant	Seawater, KB	E	MP	% recovery	106 (7)

^aExtraction efficiency controls consisted of direct extractions of microorganisms, including the liquid in which they were suspended (liquid) or after centrifuging to pellet primarily cells (*) or cells and viruses (**) as indicated (pelleted).

^bFor environmental samples, locations were Ala Wai Canal (AW) and Kāne‘ohe Bay (KB).

^cThe extraction methods refer to the standard MasterPure protocol with 15 min of proteinase K incubation and no lysozyme treatment (MP), unless otherwise indicated by 60 (60 min of proteinase K incubation), + Lys (lysozyme treatment), or LB1 (nonproprietary extraction method).

^dValues are means (standard deviations) from triplicate assays, expressed as a percentage of control values.