FIG 2.

Identification of the second promoter (T3) of hrcT gene by 5′-RACE-PCR. (A) Graphical representation of the hrpB operon. Gray or black regions represent eight genes in the hrpB operon as in Fig. 1A. The open rectangle T1 shows the location of the hrpB operon promoter upstream of hrpB1 (350 bp), and T3 shows the location of a promoter region (186 bp) upstream of hrcT. (B) Detection of the 750-bp 5′-RACE PCR product of hrcT in the T1 promoter mutant RΔBP by agarose gel electrophoresis. CK, RΔBP RNA used as a negative control. (C) Nucleotide sequence of T3 upstream of hrcT. Elements in the 5′ region include a −10 box (underlined), a potential transcriptional start site (arrow), and a putative ribosome binding site (Shine-Dalgarno [SD]). The boldface ATG represents the transcription start code of hrcT.