FIG 3.

The expression of hrcT is HrpG independent. (A) β-Glucuronidase activities of transcriptional fusions designated pT1GUS and pT3GUS in the wild-type RS105, RΔhrpG, and RΔhrpX. All strains were cultured in XOM3 medium at 28°C for 12 h, and GUS activities were determined by measuring the optical density at 415 nm using 4-MUG (4-methylumbelliferyl-β-glucuronide) as a substrate. (B) Expression analysis of hrcT and hrpF by real-time quantitative RT-PCR. RNAs were isolated from cultures of X. oryzae pv. oryzicola RS105, RΔhrpG, and RΔhrpX, which were incubated in rice suspension cells at 25°C for 16 h. Data represent the means ± standard deviations of triplicate measurements. Asterisks above bars indicate significance relative to the wild type using a paired, two-tailed Student's t test. **, P = 0.01; *, P = 0.05. The experiment was repeated three times with similar results.