FIG 7.
Expression of hrp genes and secretion of T3SEs in the hrcT-deleted mutant. (A) Symptoms induced in seedlings of rice cv. 87-15 (containing Xa27) inoculated with X. oryzae pv. oryzicola derivatives. Strains containing pHZWavrXa27 (suspended in water, OD600 = 0.3) were inoculated into leaves as described in Materials and Methods. (B) Western blot assays to examine the secretion of effector AvrXa27 using a monoclonal anti-FLAG antibody. Strains included X. oryzae pv. oryzicola RS105, RΔhrcT (hrcT deletion mutant), C1RΔhrcT and C2RΔhrcT (complemented strains of RΔhrcT with hrcT under the T1 or T3 promoter), and RΔhrcV, a T3SS mutant (negative control). With the exception of the empty vector control (pHM1), all strains contained pHZWavrXa27 (avrXa27 is fused to a Flag tag code).